Hypertension (HTN) still constitutes a worldwide problem to the healthcare section and individual’s life. The current angiotensin converting enzyme (ACE) inhibitors are first-choice option but pose deleterious side effects upon prolonged administration. The goal of the present in silico study is to evaluate the Allium sativum and Solanum lycopersicum proteins as source for anti-hypertensive natural peptides. The corresponding protein sequences were obtained from the UniProt database and then inputted to antihypertensive peptides predictor online tool. Using the same tool, 3 digestive enzymes (pepsin, trypsin and elastase 1) were chosen for the digestion of proteins into small peptides and subsequently assess their hypotensive activity. Some biochemical characteristics of positive bioactive peptides were calculated via Pepstats web interface while HAPPENN tool examined hemotoxicity. Afterwards, the secondary structure of positive bioactive peptides was de novo predicted through PEPstrMOD server prior to docking against human ACE (PDB ID: 1o86) using HPEPDOCK and ClusPro platforms. A.sativum peptides are more potent than S.lycopersicum ones in terms of IC50 as well as docking score albeit neither of which showed hemotoxicity. The present in silico work suggests the hypotensive activity of A.sativum and S.lycopersicum as natural treatment option of HTN.