Rape is a heinous crime and lot of rape crimes are registered in Maharashtra day to day. In case of a minor victim, medical examination plays important role to prove the rape. For the forensic analysis, forensic science laboratory receives the samples collected by medical officers during the medical examination of victim and accused and the other samples of them like clothes, crime scene articles seized by investigation agency. In the crime of rape, semen of accused on clothes of victim or in genital parts of victim is always the crucial scientific evidence which can prove the act of crime and can led severe punishment by law. However, every time it is not possible to obtain semen on articles as sometimes ejaculation of semen may not be achieved by the culprit. In such situations, blood also plays important role to connect the accused with the crime. We analyzed two such cases where, DNA profiles obtained from blood detected on victim’s clothes matched with DNA profiles of accused. However, as the cases were belonged to rape, accused were adult and both victims were minor, the transfer of blood of accused on their clothes was astonishing. After studying medical examination report of both the accused, it was found that they had penile injuries while attempting the crime. So, in absence of semen; the DNA profile obtained from blood on victim’s clothes found the crucial evidence in both the cases which proved the involvement of accused in the crime.