Journal of Cardiobiology
Cardioprotection Afforded by SUR2A: An Update
Sofija Jovanović, Khaja Shameem Mohammed Abdul and Aleksandar Jovanović*
- Division of Cardiovascular and Diabetic Medicine, Medical Research Institute, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK
*Address for Correspondence: Aleksandar Jovanović, Medical Research Institute, Ninewells Hospital & Medical School, University of Dundee, Dundee, DD1 9SY, Scotland, UK, Tel: +44 (0) 1382 496 269; Fax: +44 (0) 1382 632 597; E-mail: a.Jovanovic@dundee.ac.uk
Citation: Jovanović S, Mohammed Abdul KS, Jovanović A. Cardioprotection Afforded by SUR2A: An Update. J Cardiobiol. 2013;1(1): 4.
Copyright © 2013 Jovanović S, et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Journal of Cardiobiology | ISSN: 2332-3671 | Volume: 1, Issue: 1
Submission: 03 August 2013 | Accepted: 26 August 2013 | Published: 30 August 2013
AbstractSUR2A is an atypical ABC protein serving as a regulatory subunit of ATP-sensitive K+ (KATP) channels. In experimental animals, it has been found that an increase in myocardial level of this protein protects the heart against different types of metabolic stresses, including ischaemia. Increase in SUR2A leads to increase in number of fully-assembled KATP channels, which is associated with earlier channel activation during ischaemia as well as increased production of ATP by enzymes that are physically associated with the channel subunits. Activation of KATP channels shorten action membrane potential to prevent Ca2+ influx while ATP production increases subsarcolemmal ATP levels providing energy for vital energydependent processes at this localisation. The most recent findings are that SUR2A might also regulate expression of genes that are important in cardioprotection. How to increase SUR2A expression in an efficient and safe manner has been considered. Two possible approaches have been found to be promising. One is gene therapy approach with adenovirus containing SUR2A which was successful at the cellular level and the other was oral nicotinamide, a form of vitamin B3, which was efficient under ex vivo conditions. Based on all these findings, we believe that SUR2A-based strategies against heart ischaemia deserve to be further elucidated and tested. A therapy of ischaemic heart disease exploiting endogenous cardioprotective factors, including SUR2A, would be an excellent adjunct to current therapeutic strategies of ischaemic heart disease and other cardiovascular diseases where increase in cardiac resistance to stress would be beneficial.
KeywordsSUR2A; ATP-sensitive K+ channels; Cardioprotection
IntroductionIn 1986 Murry and colleagues discovered the phenomenon that is now called ischaemic preconditioning in which a brief period of blood vessel occlusion enhances the survival of cardiac cells exposed to sustained ischaemia by activating signalling pathway that increases cellular resistance to metabolic stress . It is recognised that therapy of heart ischaemia based on endogenous mechanisms of cardioprotection is warranted and that it would be an important addition to current therapeutic strategies [2,3]. In the last decade, we have focused our research on cardioprotective signalling aiming to identify possible factors of cardioprotection that could be exploited clinically. Initial findings [4-6], encouraged us to do more detailed research of ATP-sensitive K+ (KATP) channels. These channels have been originally uncovered in cardiomyocytes in 1983 . Their defining attributes are that they are selectively permeable for K+ and are regulated by intracellular ATP. As being regulated by ATP, these channels are generally viewed as a link between intracellular metabolism and membrane excitability . These channels are found in many tissues where they play vital physiological roles such as regulation of insulin secretion (in pancreatic β-cells), regulation of vascular tone and many others . Structurally, KATP channels are composed of pore-forming inward rectifiers, Kir6.1 or Kir6.2, and regulatory, ATP-binding subunits, SUR1, SUR2A or SUR2B. It is generally accepted that four Kir6.x and four SURx are physically associated with each other to form a functional KATP channel. The properties of these channels are different in various tissues due to the combinations of the subunits forming the channel. As an example, SUR1 and Kir6.2 form the pancreatic type of KATP channels, SUR2A and Kir6.2 form the cardiac type of KATP channels, and SUR2B and Kir6.1 form the vascular smooth muscle type of KATP channels. There are studies suggesting that further diversity may be generated by a combination of more than one type of Kir6.x and SURx subunits within an individual channel . It should be also pointed out that there are KATP channels localized on subcellular locations, most notable mitochondrias. The full structure and function (even existence) of these channels is still under vigorous debate .
The Mechanism of SUR2A-Mediated CardioprotectionFirst original studies on transgenic mice suggested that increased expression of SUR2A results in increased numbers of fully assembled KATP channels  resulting in earlier activation of sarcolemmal KATP channels when cardiomyocytes were exposed to metabolic stress. How and why and increase in SUR2A alone results in increased levels of KATP channels is still intriguing. One possibility is that SUR2A is the least expressed KATP channel subunit in the heart and this has been documented at the mRNA level [6,20]. Being the least expressed channel-forming protein the level of this subunit is possibly a rate limiting factor for making fully-assembled KATP channels. Indeed, an increase in Kir6.2 levels alone does not have cardioprotective properties as an increase in SUR2A alone does . Timing of activation of KATP channels in response to ischaemia in cells overexpressing SUR2A resembled closely those seen in preconditioning [15,16,20]. How increased number of KATP channels transposed into earlier activation of these channels is yet to be fully understood, but the magnitude of K+ currant flowing through KATP channels induced by increased probability of channels opening in response to ischaemia would be higher in cells expressing more channels. This means that channel probability would be 0 and >0 in cells with lower and higher channel levels respectively at the beginning of ischaemia, thus transposing to different times of increase in K+ current. The other possibility is that the higher expression of channels would result in more channels sitting near the site of ATP consumption (membrane ATPases as an example) and therefore would respond earlier to metabolic stress . The activation of KATP channels shortens action membrane potential duration and prevents influx of Ca2+ and Ca2+ overload . However, more recent studies suggested that additional mechanisms of SUR2A-mediated cardioprotection independent from the channel activity might be involved. Specifically, sarcolemmal KATP channels could produce ATP by virtue of catalytic activities of CK and glycolytic enzymes . It is possible that, during metabolic stress, ATP produced by sarcolemmal KATP channels counteracts stressinduced loss of ATP, which promotes cellular survival. It seems that maintaining subsarcolemmal levels of ATP is particularly valuable effect of KATP channels in terms of cellular protection as it provides energy at localisation where it is needed most for ion homeostasis [22,25,26]. It has been show that an increase in SUR2A expression in cardiomyocytes increases subsarcolemmal levels of ATP which is cytoprotective independently from the channels activity . Most recently, we have also found one unexpected effect of SUR2A that could have profound consequences on the cellular resistance to stress. Specifically, in experiments performed on embryonic hearts, it was shown that increased expression of SUR2A can shift cardiomycoytes towards less differentiated state by inhibiting extracellular signalregulated kinases (ERK) signalling pathway and regulating expression of different genes . Whether SUR2A has similar effect on adult cardiomyocytes is yet to be determined. However, the fact that SUR2A inhibits ERK pathway suggests that this is a real possibility. Thus, it is fair to say that, at the moment, there are 3 possible mechanisms involved in SUR2A-mediated cardioprotection that are not mutually exclusive and are likely complementary to each other:
An Increase of SUR2A in the Heart by Potentially Clinically Applicable StrategiesIn early studies, it has been shown that increase in SUR2A can be induced by estrogens or mild hypoxia [30,31]. However, both estrogens and hypoxia are not ideal for clinical use as they may be associated with adverse effects unrelated to SUR2A. In addition, estrogens would likely be efficient only in females. Therefore, we have searched for strategy of increasing SUR2A that could be clinically more applicable. The most obvious approach is gene therapy approach. Gene therapy is based on introduction of a specific gene into somatic cells in order to achieve a therapeutic effect. This concept is now well proven and the main technical obstacles in gene therapy implementation seem to be overcome. Problems with efficacy of gene delivery, safety of vectors and longevity of gene expression seems to be largely resolved and gene therapy approach in therapy of cardiovascular diseases is promising and potentially clinically viable . The most recent types of viral constructs have been developed to secure a long-term expression of a gene in the heart after a single episode of its administration . We have demonstrated that introduction of adenovirus containing SUR2A induced an increase in SUR2A expression, number of functional KATP channels and resistance to severe metabolic stress in rat heart embryonic H9C2 cell line . Furthermore, we have generated SUR2A in lentiviral vector (LVSUR2A). Lentivirus is now well established tool to secure long term expression of a gene of interest. Therefore, it seems that LV-SUR2A could be a suitable construct to achieve long-lasting expression of SUR2A in the heart. Another challenge is to establish the most suitable way to introduce LV-SUR2A construct into the heart. There are many methods for gene introduction to the heart and they need to be tested in the context of SUR2A. One promising methods of gene delivery into the heart by using coronary sinus retrograde injection employing non-invasive catheterization has been recently described. This methodology provides a widespread and uniform expression of a gene throughout the ventricular cardiac tissue and seems to be an efficient and safe method for whole heart cardiac gene transfer . Based on all these, it is quite possible that LV-SUR2A introduced into the heart by retrovenous injection in the coronary sinus employing the catheterization technique  could generate cardiac phenotype with permanently increased heart resistance to different types of metabolic stresses.
Another possible clinical strategy could be less invasive and it is based on oral nicotinamide. A decade ago, we have found that a mild chronic hypoxia up-regulates SUR2A due to changes in intracellular NADH/NAD+ ratio . These findings prompted us to consider what kind of treatment might induce a similar effect in the heart and up-regulate SUR2A in vivo. It has been reported that a nicotinamiderich diet could change the NADH/NAD+ ratio, although this has not been demonstrated in the heart . Nicotinamide is the amide form of vitamin B3 (niacin) and is obtained through synthesis in the body or as a dietary source and supplement. Nicotinic acid is the other form of the water soluble vitamin B3. We have specifically choose to test the effect of nicotinamide over more popular nicotinic acid as nicotinamide has no anti-lipid and vascular effects . If a change in intracellular NADH/NAD+ ratio up-regulates SUR2A and KATP channels in cardiac cells , then it was possible that a nicotinamide rich diet would increase myocardial SUR2A/KATP channels and, consequently, heart resistance to metabolic stress. Therefore, we have tested this hypothesis by feeding mice for a week with nicotinamide. We have measured expression of SUR2A in the heart, size of myocardial infarction in response to ischaemia reperfusion, physical endurance and survival of isolated cardiomyocytes in severe hypoxia and β-adrenergic stress [37,38]. Details of methods used were described by Sukhodub A et al. [37,38]. We have found that oral nicotinamide significantly decreases infarct size ex vivo in response to ischaemia-reperfusion, increases physical endurance and increase cellular survival to severe hypoxia and β-adrenergic stress [37,38]. The maximal effect was observed when daily intake was 2 mg of nicotinamide or below [37,38]. Nicotinamide-induced increase in the expression of SUR2A in the heart and the cardioprotective effect was largely inhibited by HMR1098, an inhibitor of sarcolemmal KATP channels , and in mice unable to form KATP channels . The maximal effective cardioprotective dose in mice we used is the dose that is equivalent to 500 mg/day of nicotinamide in people or less (the human equivalent dose was calculated using mouse dose and dose translation according to Reagan-Shaw S et al. ). Nicotinamide has a great potential as a SUR2A-based therapeutic as it is known to be one of the safest drugs in clinical practice. It should not be confused with nicotinic acid (also known as niacin, vitamin B3 and vitamin PP) that is also a vitamin B3 form but exhibits more pharmacological and adverse effects than nicotinamide . Niacin (nicotinic acid) is known to increase levels of HDL cholesterol, which has been suggested to decrease the risk of cardiovascular events. However, in a recent trial AIM-HIGH, a slow-release form of niacin, used for its effect on lipids, was found to have no effect on cardiovascular event and the trial was halted prematurely on evidence that niacin addition to statins actually increased stroke risk in this group . It is important to point out that in contrast to nicotinic acid nicotinamide has no lipid and vascular effects, that it is not a compound used in AIMHIGH trial and no similar effect of nicotinamide would be expected . Thus, cardioprotection afforded by nicotinamide has never been tested in vivo so far. Current therapeutic use of nicotinamide is confined to treatment of acne vulgaris, diabetes mellitus and head and neck cancer, all of which are in doses above 500 mg/day . It has been suggested that chronic intake of nicotinamide below 3000 mg/day is safe and virtually devoid of adverse effects . Therefore, chronic treatment with 500 mg/day of nicotinamide is likely to be safe. At the same time, it is realistically to expect that nicotinamide taken orally 500 mg/day would significantly increase myocardial resistance to ischaemia. As contraindications and drugs interactions of nicotinamide are rare, almost any patient suffering from IHD on any therapy could use oral nicotinamide for his/her clinical benefit.
Conclusions and Future PerspectivesIncrease in expression of SUR2A increases heart resistance towards different types of metabolic stresses, including ishaemia and ischaemia-reperfusion in different animal experimental models. Gene therapy and nicotinamide were identified as two ways of increasing myocardial SUR2A that could be clinically applicable. More animal studies are required to fully establish the mechanism underlying SUR2A-mediated cardioprotection and elucidate in vivo efficacy and safety. Such work would provide rationale and justification for clinical trials that would test a clinical value of SUR2A-based therapy.
AcknowledgementsResearch described in this review was supported by grants from BBSRC, British Heart Foundation, Wellcome Trust, MRC, TENOVUS-Scotland and Anonymous Trust, UK.
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