Assessment of Vitamin D Level in Patients of Alopecia Areata

It was said that the development of hair follicle depends on the Vitamin D Receptor (VDR) expression which correlates with increased differentiation of the follicle keratinocytes [7]. The increased incidence of AA let us to search more for possible etiological factors. The relation between vitamin D deficiency and AA was mentioned recently in previous literature, so we try to confirm these results and see if there is possible relation between vitamin D deficiency and the pattern of AA.


Introduction
Alopecia Areata (AA) is an autoimmune disease which is characterized by hair loss and can affect any hair-bearing area.Clinically, AA present with different clinical manifestations varying from reversible patchy hair loss to complete baldness or complete body hair loss.It affects approximately 1-2% of the general population [1].
Features of AA are caused by CD4+ and CD8+ T cells targeting hair follicles as an autoimmune-mediated skin disease attacks the hair follicle [2].
Vitamin D has been found to have immunoregulatory effects.1,25(OH)2D3, which is the major form of vitamin D, is a modulator of immune functions including activities of T-lymphocytes and B-lymphocytes as well as having role on immune responses [4].
The concentration of 1,25(OH)2D3 depends on an adequate supply of circulating 25(OH)D which is described as the primary indicator of vitamin D status [5].
Vitamin D deficiency was established as a risk factor for occurrence of some autoimmune diseases as SLE, Psoriasis and vitiligo [6].
It was said that the development of hair follicle depends on the Vitamin D Receptor (VDR) expression which correlates with increased differentiation of the follicle keratinocytes [7].
The increased incidence of AA let us to search more for possible etiological factors.The relation between vitamin D deficiency and AA was mentioned recently in previous literature, so we try to confirm these results and see if there is possible relation between vitamin D deficiency and the pattern of AA.
Alopecia Areata (AA) is a T cell-mediated autoimmune disease that causes inflammation around anagen-stage hair follicles.Insufficient levels of vitamin D have been implicated in a variety of autoimmune diseases.Previous reports have described the effects of vitamin D on hair follicles.To evaluate the status of vitamin D in patients with AA, and the relationship between vitamin D levels and disease severity, a cross-sectional study of 23 patients with AA and 23 healthy controls was conducted.Serum vitamin D levels of the study group were determined by ELISA technique.Results revealed a significant difference between cases and control as regards 25(OH) vitamin D level and its status.There is also a significant difference between 25(OH) vitamin D status and the degree of AA but not with the pattern of AA.However, there is a non-significant difference in the 25(OH) vitamin D status in all participants regarding age and sex of subjects.Accordingly, screening patients with AA for vitamin D deficiencies seems to be of value for the possibility of supplementing these patients with vitamin D. • Mild AA: patients who were S1 (<25% hair loss)

Statistical analysis:
• The collected data were computerized and statistically analyzed using SPSS program (Statistical Package for Social Science) version 18.0.
• Chi square test was used to calculate difference between qualitative variables.
• Quantitative data were expressed as mean ± SD (Standard deviation).
• Independent T test was used to calculate difference between quantitative variables in two groups.
• ANOVA F-test was used to calculate difference between quantitative variables in more than two groups.
• Pearson correlation coefficient used to calculate correlation between quantitative variables.

Results
No significant difference between cases and controls regarding age and sex of subjects, Table 2. Results revealed a significant difference between cases and control as regards 25(OH) vitamin D level and its status, Table 3.Also, there is a significant difference between 25(OH) vitamin D status and the degree of AA but not with the pattern of AA, Table 4.However, there is an on-significant difference in the 25(OH) vitamin D status in all participants regarding age and sex of subjects Table 5.

Discussion
Alopecia areata is an organ-specific autoimmune disease characterized by T-cell infiltrates and cytokine production around anagen-stage hair follicles [10].
Autoimmunity in AA is strongly supported by clinical observations that patients with AA are often diagnosed with one or more other autoimmune disorders such as vitiligo, lupus erythematosus and Type I diabetes.In addition, the efficacy of various immunosuppressive agents including cyclosporine and systemic corticosteroids also suggest anautoimmune mechanism.Further, the Human Leukocyte Antigens (HLA) has been reported to play a major role in the etiology of autoimmunity.This specific hypothesis is confirmed by the increased expression of specific HLAs in AA patients such as HLA-DR, HLA-A, HLA-B, and HLA-C, which are rarely seen in healthy individuals as well as the identification of a number of genetic risk factors within various innate and adaptive immunity gene loci [11].Cytokines including IL-1α, IL-1β and TNF-α may play an important role in the pathophysiology of AA.CD8+ T cells are considered to act as the effector cells with help from CD4+ T cells [12].
Vitamin D is a steroid hormone that plays an important role in calcium homeostasis and bone health.Its main source is endogenous synthesis in the skin which is induced by ultraviolet B radiation.It can also be obtained exogenously through the diet [13].In the immune system, 1,25(OH)2D3 targets various immune cells, especially T lymphocytes and B lymphocytes, cytokines and modulates both innate and adaptive immune response [7].
It was said that VDR may play a vital role in the postnatal maintenance of the hair follicle.It is expressed in dermal papillae cells and in the epidermis of the hair follicle in varied degrees in correlation with the stages of the hair cycle [14].
A recent study demonstrated that the decreased expression of VDR in AA and alopecia universalis lesions is related to decreased expression of Wnt signaling pathway, which inhibits proliferation and differentiation of hair follicles and epidermal cells [15].
A recent systematic review found that vitamin D seems to play a beneficial role in the prevention of autoimmune disorders [16].Organz-Molina et al. found a decreased level of vitamin D in patients with psoriasis [17].While Beheshti et al. found a decreased level of vitamin D in patients with vitiligo [18].
The aim of this study was to evaluate the relation of Vitamin D in the pathogenesis of AA which may be helpful in its treatment.
The present work was performed on 23 patients with AA and 23 healthy controls to detect their serum 25(OH)D level.
The results showed a statistically significant decrease of mean serum 25(OH)D level among patients of AA (14.13±8.72 ng/ml) compared to control group (22.43±10.94ng/ml) with (P value=0.01).
Our results are parallel with the study made by (Yilmaz et al.) which showed a statistically significant difference between AA patients (n=42) and control group (n=42) regarding serum D concentration (P<0.001) which suggested that vitamin D deficiency may have a role in the setting of AA [7].Another study by Nassiri et al. investigated serum 25(OH)D level of 28 patients with AA and 44 healthy controls [19].The median serum 25(OH)D was lower in patients with AA than controls although the difference was not statistically significant (P=0.07), also El-Mongy et al. ran a study on 70 cases of AA and 70 healthy controls, the study showed that serum 25(OH)D levels was significantly decreased in AA patients than in controls (P=0.023)[20].
Our results support the results of the study by Mahamid et al. who investigated 23 patients with AA and 23 healthy controls for serum 25(OH)D level [21].A strong correlation between AA and vitamin D deficiency was found (P=0.02).This study also found an elevated C-reactive protein levels in the patients group which suggests inflammatory mechanisms apart from the proposed immune mechanism.
Cerman et al. investigated serum 25(OH)D level in 86 patients with AA, 31 patients with vitiligo and 58 healthy controls [12].The prevalence of 25(OH)D deficiency was significantly higher in the group of patients of AA (P=0.001) than in the group of patients of vitiligo (P<0.001) compared with the group of healthy controls.These results link between the low vitamin D level and the occurrence of various autoimmune diseases such as AA, Vitiligo, SLE, DM, and Rheumatoid Arthritis.On the other hand, a study by D'Ovidio et al. made in Italy demonstrated the presence of insufficient level of 25(OH)D in patients with chronic and relapsing AA [22].This study was done on 156 patients of AA and 148 healthy controls.The results confirmed that the level of vitamin D in AA patients was lower than controls although the results were not statistically significant (P=0.24).This may be due to the universal tendency to lower values of 25(OH)D even in the normal population in Italy due to lack of sun exposure.
In the present study, an insufficient level of vitamin D was more common in females (70.6%) than in males (48.3%), but it wasn't statistically significant, may be because the female participation in this study was quite limited (34% of cases and 39% of controls were females).
In the study of El-Mongy et al. the female participants in both cases (n=33) and controls (n=26) showed a significant lower vitamin D level in compared with male participants in cases (n=37) and controls (n=44) with a P value equaled (0.005) [19].The same was noted in Cerman et al. with a P value equaled (0.041) this low vitamin D level in females may be attributed to its consumption in pregnancy and lactation, and some social and cultural factors as the conservative dress of Egyptian women blocks exposure to sunlight which is the main source of vitamin D synthesis [12].
In the present study AA was classified to mild, moderate, and severe degrees according to (SALT) score.By comparing serum 25(OH)D status in the patients in relation to degree of AA, we found a statistically significant difference between vitamin D status and degree of AA (P=0.02).This result met with the result of Cerman et al. who reported a significant negative correlation between the degree of AA by (SALT) score and serum 25(OH)D level in AA patients (P<0.001)[12].Also, a more lowering of vitamin D level (below 10 ng/ml) was noted in patients with extensive AA (Alopecia totalis and Alopecia universalis) when compared with its level in patients with patchy AA but this difference was not statistically significant.This may be due to limited number of patients with Alopecia totalis (n=2) and Alopecia universalis (n=1).This finding may support the role of vitamin D in the evolution of AA.
Conversely, D'Ovidio et al. found no correlation between the severity of AA and serum 25(OH)D level [21].This may be because methodological data such as the scoring of AA are not available in this study.
Regarding age, in the present study there was a tendency for the presence of a lower serum 25(OH)D level in the older age group (from 41-50 yrs) in all participants when compared to the younger age groups.
This may be because with increasing age, solar exposure is usually limited because of changes in lifestyle factors such as clothing and outdoor activity.Diet may also become less varied.Most importantly, however, the dermal production of vitamin D following a standard exposure to UVB light decreases with age because of atrophic skin changes with a reduced amount of its precursor.Also, the renal production of 1,25(OH)2D decreases because of diminishing renal function with age [23].
All of these findings points to the important role of vitamin D in the pathogenesis of AA and must be estimated in all patients of AA especially the females and the old patients which may open a new channel for its treatment.
hair loss Classifications of patients were classified regarding the severity of AA according to SALT score into: D status: Vitamin D status was classified according to serum 25(OH) vitamin D concentrations into: • Vitamin D deficiency: 25 OH D concentration <10 ng/ml.• Vitamin D insufficiency: 25 OH D concentration 10-30 ng/ml.• Vitamin D sufficiency: 25 OH D concentration >30 ng/ml.

Table 1 :
Medical history of cases.This table shows medical history of cases regarding duration of the disease, family history, degree and pattern of AA.

Table 2 :
Socio-demographic characters of controls and cases.

Table 3 :
Comparison between cases and controls regarding serum 25(OH)D status and level.